Advanced Fertility Procedures

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Sarvodaya Women's Hospital And Fertility (IVF) Centre

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These techniques are often used during IVF treatment to increase the chances of success or preserve fertility for the future. Let’s go through each one

Blastocyst Culture

What is it?

After eggs are fertilized in the lab, embryos usually grow for 3–5 days. When we allow them to grow for 5 days, they reach a stage called a blastocyst — a more advanced embryo.

Why is it done?

Blastocysts are stronger and more likely to implant successfully.
It helps choose the best embryo for transfer.
Useful if you’re planning genetic testing or embryo freezing.

Laser-Assisted Hatching

What is it?

An embryo is surrounded by a thin outer shell called the zona pellucida. In some cases, this shell is harder or thicker than usual.
A gentle laser is used to make a tiny opening in this shell to help the embryo “hatch” and implant into the uterus.

Why is it done?

May improve chances of pregnancy, especially:
- In women over 35
- In frozen embryo transfers
- In previous IVF failures

Embryo Biopsy (for Genetic Testing)

What is it?

A few cells are safely removed from an embryo (usually at the blastocyst stage) without harming it. These cells are tested for genetic or chromosomal conditions.

Why is it done?

To check for inherited disorders (like thalassemia or cystic fibrosis)
To avoid transferring embryos with abnormalities
Often used before PGT-A, PGT-M, or PGT-SR

Note: The embryo is usually frozen after the biopsy, and transfer is planned in a later cycle.

Microsurgical Sperm Extraction (PESA / TESA / TESE / Micro-TESE)

What is it?
When sperm cannot be collected naturally (e.g., due to blocked or absent sperm ducts), sperm is retrieved directly from the testicles using a minor surgical procedure.

Types include

PESA: From the epididymis using a fine needle

TESA/TESE: From the testicle with a needle or small biopsy

Micro-TESE: A more advanced version using a microscope to find sperm

Embryo Cryopreservation (Freezing)

What is it?
Good-quality embryos that are not used in the first transfer can be frozen for future use. This is called embryo cryopreservation.

Why is it done?

Saves extra embryos for future pregnancy attempts
Avoids the need for repeated egg collection
Allows time for genetic testing or health recovery before transfer

Frozen embryos can be stored for many years and used in a frozen embryo transfer (FET) later on.

Sperm, Egg (Oocyte), and Gonadal Tissue Freezing

What is it?

Sperm freezing: Semen is collected and frozen for future use — often used before surgery, cancer treatment, or travel.
Egg freezing (Oocyte cryopreservation): Healthy eggs are collected and frozen, often for social or medical reasons (e.g., before cancer treatment or delaying childbearing).
Gonadal tissue freezing: Ovarian or testicular tissue is frozen — often used in children or young adults before cancer treatment when egg or sperm collection isn’t possible.

Why is it done?

To preserve fertility for the future
To give more flexibility in family planning
To allow fertility after cancer or other treatments

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